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Image Search Results
Journal: Frontiers in Chemistry
Article Title: Lotus seed (Nelumbinis semen) extract: anticancer potential and chemoprofiling by in vitro , in silico and GC-MS studies
doi: 10.3389/fchem.2024.1505272
Figure Lengend Snippet: Annexin-V FITC/PI staining of apoptosis at 24 h of incubation. (A) SKOV3-CisR control, (B) A2780-CisR cells, (C) A2780-CisR control, (D) MELS treated SKOV3-CisR cells.
Article Snippet: Two human ovarian cancer cell lines, namely,
Techniques: Staining, Incubation, Control
Journal: Frontiers in Chemistry
Article Title: Lotus seed (Nelumbinis semen) extract: anticancer potential and chemoprofiling by in vitro , in silico and GC-MS studies
doi: 10.3389/fchem.2024.1505272
Figure Lengend Snippet: Flow cytometric cell cycle distribution analysis. (A) SKOV3-CisR control cells, (B) MELS treated SKOV3-CisR, (C) Control cells of A2780-CisR, (D) MELS treated A2780-CisR cells.
Article Snippet: Two human ovarian cancer cell lines, namely,
Techniques: Control
Journal: Cancers
Article Title: Clinical Value of lncRNA MEG3 in High-Grade Serous Ovarian Cancer
doi: 10.3390/cancers12040966
Figure Lengend Snippet: MEG3 modulation affected proliferation and clonogenic capability of high-grade serous ovarian cancer (HGSOC) cells. ( A ) Relative MEG3 expression and ( B ) relative MEG3 subcellular localization assessed by RT-qPCR analysis in a panel of HGSOC cell lines as well as in HOSE (Human ovarian surface epithelial cells) and FT194 (Fallopian Tube epithelial cells). Data from total RNA are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using FT194 as a reference sample. Relative MEG3 expression in nucleus vs. cytoplasm is expressed as Log2FC values calculated with the ΔCt method and compared to FT194 cells; positive values represent nuclear enrichment. ( C ) Relative MEG3 expression assessed by RT-qPCR analysis in HEY and PEO1 cells (HGSOC cell lines) transiently transfected with pMEG3 (MEG3 expression plasmid) and with pcDNA (empty vector). Data are presented as Log2 fold change (Log2FC) values calculated with the ΔΔCt method, using pcDNA as reference sample. ( D ) Bar chart representing proliferation assay for HEY-pMEG3 and PEO1-pMEG3 cells compared to respective control cells. Viable cells were counted at 24, 48, and 72 h from transfection. The mean cell proliferation at time x (Tx) was expressed as average percentage increase relative to T = 0 h (T0). ( E ) Clonogenic assay. Bar charts represent differences of clonogenic capability in MEG3 overexpressing cells with respect to control cells and representative pictures of clonogenic assays. For all experiments, bars and error bars refer to mean and SEM (standard error of the mean) of three experiments. To establish statistically significant differences, unpaired t -test was carried out: * p < 0.05; ** p < 0.01.
Article Snippet: The immortalized human
Techniques: Expressing, Quantitative RT-PCR, Transfection, Plasmid Preparation, Proliferation Assay, Control, Clonogenic Assay